Biophysical Study of Human Serum Albumin upon Interaction with La2O2CO3 Nanoparticles
کد مقاله : 1050-PHYSCHEM20
سمیه شهرکی *
چکیده مقاله:
The production and utilization of nanomaterials unavoidably lead to their accumulation in the environment, thereby posing a threat to human and environment. These compounds go into the human body, soil, or water. The interaction between La2O2CO3 nanoparticles and human serum albumin (HSA) was studied by using spectroscopic measurements. The apparent association constant has been deduced (Kapp = 1.1 × 103 M-1) from the absorption spectral changes of HSA–La2O2CO3 nanoparticles. Addition of La2O2CO3 nanoparticles effectively quenched the intrinsic fluorescence of HSA. The number of binding sites (n = 1.11) and apparent binding constant (K = 3.1 × 104 M-1) were calculated by relevant fluorescence data. The interaction between La2O2CO3 nanoparticles and HSA occurs through static quenching mechanism. The effect of La2O2CO3 nanoparticles on the conformation of HSA has been studied by means of FT-IR and UV–visible absorption spectra.
کلیدواژه ها:
La2O2CO3 nanoparticles, Fluorescence quenching, Human serum albumine
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