Studying of Electron Transfer Rate Constant of Cytochrome c Immobilized on Nanoparticle Decorated Hybrid System by Cyclic Voltammetry Technique
کد مقاله : 1102-PHYSCHEM20
ولی علی زاده *، احمد جمالی مقدم
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چکیده مقاله:
Abstract: A hybrid of cytochrome c (cyt c) and a 4-5 nm gold nanoparticle (AuNP) on Au electrode (AuE) was prepared. In the hybrid system, AuNPs was attached to the AuE via 1,6 hexandithiol (1,6 HDT) monolayer and capped with captopril (capt) molecule then electrostatically conjugated with cyt c. The AuE/AuNP/cyt hybrid system was characterized by cyclic voltammetry (CV). CV shows a very closely ideal protein voltammetric response with low peak separation in both low and very high scan rates. It is suggested that on curved AuNPs, the density of immobilized cyt c smaller than AuNP free monolayer and resultant low electrostatic repulsion between cyt c molecules during reduction or oxidation. Moreover, enhancement of the interfacial electron transfer (ET) rate of AuE/AuNP/cyt system compared with capt/cyt c was observed. This study gave fast rate constants, 1×109cm3mol-1s-1 for bimolecular and 69.6 s-1 for tunneling ET rate constants.
کلیدواژه ها:
Keywords: Cytochrome c, Gold nanoparticle, Captopril, Cyclic Voltammetry, Electron transfer Rate Constant.
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