Bovine Serum Albumin interactions with Schiff base study by spectroscopic techniques
کد مقاله : 1148-PHYSCHEM20
نویسندگان:
شبنم جم پور کلور *
دانشجوی کارشناسی ارشد
چکیده مقاله:
A new Schiff-base compound Ternary Cu (II) complexes of Schiff - base tyrosine amino acid derivatives and phnan trotline hetrocycle (A) was synthesized and the interaction between this Schiff base and bovine serum albumin (BSA) was investigated by fluorescence and Fourier transform infrared spectroscopies. A marked decrease in the fluorescence intensity of BSA was observed upon addition of Schiff base when excitation wavelength was set at 295nm. Based on the fluorescence quenching calculation, the quenching constant (Kq), apparent quenching constant (Ksv), binding constant (Kb) and binding site number (n) were obtained. The quenching mechanism was suggested as static quenching according to the Stern–Volmer equation. In addition, .the infrared spectra of BSA in the absent and present of Schiff base was studied.
کلیدواژه ها:
Schiff base; BSA; Fluorescence; FTIR; quenching constant
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