Biophysical Studies on the Interaction of Cationic Urethane Gemini Surfactant with Insulin
کد مقاله : 1194-PHYSCHEM20
1پونه سادات پورحسینی *، 2Rezvaneh Ghasemi Tabesh، 1Ali Akbar Saboury، 1فرهود نجفی
1هیأت علمی
2دانش آموخته
چکیده مقاله:
The interaction of cationic urethane gemini surfactant, having two methylene units in spacer (C2), with insulin was studied in phosphate-buffered saline PBS, pH 7.4, at 25 C as a function of surfactant concentration. The investigation dealt with fluorescence spectroscopy, in which the Stern-Volmer plot of acrylamide quenching experiment was studied. The values of F0/F versus acrylamide concentration were depicted, where F0 and F were the fluorescence intensity in the absence and presence of acrylamide. Results show that the slope of the Stern-Volmer plot decreases continuously upon increasing the surfactant concentration. This observation indicates the progressive restricted accessibility of tyrosines to the environment, which is the consequence of either increasing the helical content of the protein or shielding effect of the hydrophobic interior of the nanomicelles.
کلیدواژه ها:
Insulin; acrylamide; fluorescence spectroscopy; quenching
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