Lipase Immobilization on Metal-Organic Framework and Evaluation of Initial Enzyme ‎Concentration Effect
کد مقاله : 1290-PHYSCHEM20
عاطفه زارع *، عبدالخالق بردبار
دانشگاه اصفهان
چکیده مقاله:
In the study, one of the metal-organic frameworks, MIL-101-NH2 (Cr), was synthesized by solvothermal method and ‎functionalized with 2,4,6-trichloro-1,3,5-triazine (TCT). Amino MIL-101(Cr) and its chemical functionalized form ‎‎(TCT@NH2-MIL-101(Cr)) were used as support for candida rugose lipase (CRL) immobilization. The effect of initial ‎CRL concentration on the enzyme loading and the immobilized ‎enzyme activity were investigated‎. The result ‎indicated that with increasing the amount of enzyme loading, the corresponding specific activity decreases. The ‎amount of enzyme loading was determined by Bradford method. The catalytic activity of the immobilized lipase was ‎assayed by the hydrolysis of p-nitrophenyl palmitate (p-NPP). FT-IR spectra, XRD diffraction and FE-SEM analysis ‎were used to confirm successive synthesis, functionalization and immobilization. ‎
کلیدواژه ها:
Lipase, Immobilization, Metal-organic framework ‎
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