Partitioning of L-tryptophan in aqueous biphasic systems composed of PEG 4000 and some biodegradable salts at T = 298.15 K
کد مقاله : 1302-PHYSCHEM20
ملیحه فردشیرایه *
چکیده مقاله:
Partitioning in aqueous biphasic systems (ABS) is widely recognized today as a rapid, gentle, and highly efficient technique for the separation of soluble as well as particulate biomaterials. This technique has gained increasing attention as the separation method of choice in biotechnology. Here, the partitioning behaviour of the amino acid L-tryptophan between the two phases of different PEG-based ABS composed of PEG 4000 and various biodegradable salts such as tri-sodium citrate (Na3C6H5O7), di-sodium tartrate (Na2C4H4O6), or di-sodium succinate (Na2C4H4O4) was examined. The partition coefficients of L-tryptophan (KTrp) were determined in the studied systems at T = 298.15 K. It seems that in addition to phase hydrophobicity effect, the salt ability to salting-out amino acid can be one of the major factors for transferring amino acid to PEG-rich phase.
کلیدواژه ها:
Aqueous biphasic system; Polyethylene glycol; partitioning; L-tryptophan
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